Summary
Type: SPE
Application #: 304070
Catalog#: 730002 - SPE Cartridges, Chromabond, C18, 3ml, PP, 200mg, 50/pk
Detection: GC/MS after derivatisation with pyridine-acetic anhydride on e.g. Optima® 1
Conditions: Column conditioning: The column was conditioned with 2 ml of methanol and then with 1 ml of deionised water at a flow rate of 30 ml/min.
Sample aspiration: The mixture was passed through the column.
Column washing: The column was rinsed with 1 ml of deionised water and 1 ml of a 50 g/L NaHCO3 solution, followed by 1 ml of deionised water at a flow rate of 30 ml/min. The column was then dried with nitrogen (300kPa) for 5 min.
Elution: The analyte was eluted with 3 ml of dichloromethane-acetone (1 ml of dichloromethane + 3 ml of acetone) at 6 ml/min.
Evaporation: The extract was evaporated to dryness.
Authors: Toennes SW Maurer HH
Source: Clinical Chemistry 45:12 2173-2182 (1999)